While the former is a normal process in some green plants, the latter is an abnormal and injurious process occurring in extremely intense light resulting in destruction of cellular components, cells and tissues. These are minimum, optimum and maximum. The vascular bundles in C4 plant leaves are surrounded by a layer of bundle sheath cells that contain large number of chloroplast. However, it is possible to show that photosynthesis consists of a combination of light-requiring reactions (the “light reactions”) and non-light requiring reactions (the “dark reactions”). During photosynthesis water is oxidized and carbon dioxide is reduced, but where in the overall process light energy intervenes to drive the reaction. It is usually constant for a plant species but rarely it varies. 6. In other words, 75% of the carbon lost by oxygenation of RuBP is recovered, and 25% is lost as release of one molecule of CO2. 1. (ii) Stroma is more developed but granna is poorly developed. 2. The concentration for oxygen in the atmosphere is about 21% by volume and it seldom fluctuates. 2. Along with the NADPH produced by the movement of electrons, the ATP will be used immediately in the biosynthetic reaction taking place in the stroma, responsible for fixing CO2, and synthesis of sugars. The bacteria after few minutes aggregated more in that regions which were exposed to blue and red wave length. When the intensity of light is low, the reaction is limited by the small quantities of reduced coenzymes available so that any increase in temperature has little effect on the overall rate of photosynthesis. Their main function is to harvest light energy and transfer it to their respective reaction centre. These are of two of types viz., Carotene and Xanthophyll (Carotenol/Xanthol). and arrangement of leaves. From this triplet state excited molecule may return to ground state in three ways-by losing its extra energy in the form of heat, by losing extra energy in the form of radiant energy is called phosphorescence. Other inhibitors of photosynthesis are monouron or CMU (Chlorophenyl dimethyl urea), diuron or DCMU (Dichlorophenyl dimethyl urea), bromocil and atrazine etc., which have the same mechanism of action as that of violates. It is now clear that tall the reactions for the incorporation of CO2 into organic materials (i.e., carbohydrate) can occur in the dark (the “dark reactions”). It is the phenomenon of re-radiation of absorbed energy. Discussion in ' CBSE Class 7 Science Help ' started by g mahesh , Nov 7, 2013 . Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that can later be released to fuel the organisms' activities. This means that the reactants, six carbon dioxide molecules and six water molecules, are converted by light energy captured by chlorophyll (implied by the arrow) into a … 3. Radiation longer than those of red are called infra red (760 – 10,000 nm). These are insoluble in water and precursor of Vitamin A. Photorespiration is the light dependent process of oxygenation of RUBP (Ribulose bi-phosphate) and release of carbon dioxide by photosynthetic organs of the plant. (CAM was for the first time studied and reported by Ting (1971). Glycolytic reversal phase (sugar formation phase). Calvin along with A.A. Benson, J. Bassham used radioactive isotope of carbon (C14) in Chlorella pyrenoidosa and Scenedesmus oblique’s to determine the sequences of dark reaction. It converts CO 2 into carbohydrate which involves the reduction of carbon with H 2 O as reductant. The process of photosynthesis requires solar energy, water and carbon dioxide. According to Arnon, the functional relationship between the “light” and “dark” reactions can be established by examining the requirements of the dark reactions. Green plants build themselves using photosynthesis. The atmosphere normally contains 0.03 to 0.04 per cent by volume of carbon dioxide. What is chlorophyll? Why are we so interested in the proton gradient? g mahesh Member The end of disc shape thylakoid is called as margin and the area where the thylakoids membranes are appressed together is called partition. For instance: (i) It has been observed that the rate of a process cannot be increased indefinitely by increasing the availability of all the known factors; (ii) The principle of Blackman is not operative for toxic chemicals or inhibitors and. Photosynthesis is the most important natural process which sustains life on earth. (vii) CO2 acceptor is PEPA (3C) but absence of RUBP. This website includes study notes, research papers, essays, articles and other allied information submitted by visitors like YOU. (iii) Only PS-I present but absence of PS-II. The temperature affects the velocity of enzyme controlled reactions in the dark stage. Since the synthesis of food occurs in the presence of sunlight, it is called photosynthesis. These resources were hosted on the Chemistry for Biologists website, which launched in 2004 and was supported by the Royal Society of Chemistry and the Biochemical Society. PAN is known to inhibit Hill’s reaction. ! This cycle requires reducing power in the form of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and chemical energy in the form of adenosine triphosphate (ATP). 6.17). 5. What is the… Get the answers you need, now! The environmental factors which can affect the rate of photosynthesis are carbon dioxide, light, temperature, water, oxygen, minerals, pollutants and inhibitors. This gradient triggers the coupling factor to synthesize ATP from ADP and inorganic phosphate (Pi). A more specific effect of drought on photosynthesis results from dehydration of protoplasm. (vi) Stroma carries PEPCO but absence of RuBisCO. Diquat and Paraquat (commonly called as Viologens) block the transfer of electrons between Q and PQ in PS II. If light is given to granna fraction in the presence of suitable hydrogen acceptor and in complete absence of carbon dioxide then assimilatory power, ATP and NADPH2, are produced. In the peroxisome, the glyoxylate is used to form the amino acid, glycine. 5. Give a brief description of the process of synthesis of food in green plants. It synthesizes organic food from inorganic raw materials. Internal or genetic factors are all related with leaf and include protoplasmic factors, chlorophyll contents, structure of leaf, accumulation of end product etc. If photosynthesis is allowed to proceed in presence of CO218 and normal water then heavy oxygen is not evolved. In the process, cells release CO2. Functional Relationship between Light and Dark Reactions: Emerson Red Drop Effect and Enhancement Effect: Evidence in Support of Two Phases of Photosynthesis: II. You may need to download version 2.0 now from the Chrome Web Store. C4 plants have greater rate of carbon dioxide assimilation than C3 plants because PEPCO has great affinity for CO2 and it shows no photorespiration resulting in higher production of dry matter. Cyclic photophosphorylation is performed by photosystem I only. This factor affect the dark reactions. The carbon dioxide fixation takes place in the stroma of chloroplasts because it has enzymes essential for fixation of CO2 and synthesis of sugar. Give a brief description of the process of synthesis of food in green plants Share with your friends. Rate of photosynthesis is independent of duration of light. Ask your question. Aggregation of thylakoids to form stacks of coin like structures known as granna. Photosynthetic pigments are grouped into 3 categories: These are green coloured most abundant photosynthetic pigments that play a major role during photosynthesis. This 4-C acid (OAA) enters into the chloroplast of bundle sheath cell and there it undergoes oxidative decarboxylation yielding pyruvic acid (3C) and CO2. The carbon dioxide released in bundle sheath cell reacts with RuBP (Ribulose 1, 5 bisphosphate) in presence of RUBISCO and carry out Calvin cycle to synthesize glucose. This mechanism happens in chlorophyll a (Universal Photosynthetic Pigment). Because of the need for NADPH2 as a reductant and ATP as energy equivalent, CO2 fixation is closely linked to the light reactions. Chlorophyll is a substance in … Maximum is that magnitude of a factor beyond which the process stops. Photosynthesis is the process in which green plants use sunlight to make their own food. In C4 cycle two carboxylation reactions take place. The other portion is called F1 and protrudes on the outer surface of the thylakoid membrane on the side that faces the stroma. The same is extruded out when the reaction centre absorbs light energy (hv). Performance & security by Cloudflare, Please complete the security check to access. The oxygenation of RuBP in the presence of O2 is the first reaction of photorespiration, which leads to the formation of one molecule of phosphoglycolate, a 2 carbon compound and one molecule of phosphoglyceric acid (PGA). Physical Separation of Chloroplast into Granna and Stroma Fraction: It is now possible to separate granna and stroma fraction of chloroplast. The electron is handed over to reaction centre P700 of PS-I by plastocyanin. From the chloroplast, the glycolate is diffused to peroxisome, where it is oxidised to glyoxylate. When the amount of light absorbed by a pigment is plotted as a function of wave length, we obtain absorption spectrum (Fig. While passing between ferredoxin and plastoquinone and/or over the cytochrome complex, the electron loses sufficient energy to form ATP from ADP and inorganic phosphate. Photorespiration is initiated under high O2 and low CO2 and intense light around the photosynthesizing plant. Sachs (1860) recognized three critical values, the cardinal values or points of the magnitude of each factor. This time these are membranes of the thylakoid. Hill observed that the chloroplasts extracted from leaves of Stellaria media and Lamium album when suspended in a test tube containing suitable electron acceptors (Potassium feroxalate or Potassium fericyanide), Oxygen evolution took place due to photochemical splitting of water. It contains cp-DNA (0.5%), RNA (2—3%), Plastoribosome (70S), enzymes for carbon dioxide assimilation, proteins (50—60%), starch grains and osmophilic droplets, vitamin E and K, Mg, Fe, Mn, P, etc. Out of the total solar energy reaching to the earth, about 2% is used in photosynthesis and about 10% is used in other metabolic activities. Photosynthesis consists of two stages, the light reactions, and the dark reactions. Plants can actively exhibit photosynthesis under continuous light without being damaged. The idea of Van Neil was supported by R. Hill. (b) Noncyclic Photophosphorylation (Z-Scheme) (Fig. At this stage, some other factor became limiting. The size of a quantasome is about 18 x 16 x l0nm and found in the membrane of thylakoids. CO2 enters the leaf through stomata. It is a double membranous cytoplasmic organelle of eukaryotic green plant cells. (a) Since splitting of the water molecule takes place on the inner side of the membrane, the protons or hydrogen ions that are produced by the splitting of water accumulate within the lumen of the thylakoids. The value of CO2 compensation point is very low in C4 plants (0-5 ppm), where as in C3 plants it is quite high (25-100 ppm). Photolysis of Water and Oxygen Evolution (Hill Reaction): Before 1930 it was thought that the oxygen released during photosynthesis comes from carbon dioxide. If then, light is made available, the synthesis starts again. It also affects the opening and closing of stomata thereby affecting the gaseous exchange. Under normal conditions water rarely seems to be a controlling factor as the chloroplasts normally contain plenty of water. LHC comprises antenna pigments associated with proteins (viz.., antenna complex). Therefore, scientists are busy in genetically manipulating the crops. Photosynthesis requires sunlight, chlorophyll, water, and carbon dioxide gas. Since photorespiration requires additional energy from the light reactions of photosynthesis, some plants have mechanisms to reduce uptake of molecular oxygen by Rubisco. These vacuoles are used for storing malic and other acids in large amounts. The PGA molecule enters the calvin cycle to make carbohydrates, but one CO2 molecule released in mitochondria during photorespiration has to be re-fixed. The reaction results in the formation of a temporary 6 carbon compound (2-carboxy 3-keto 1,5-biphosphorbitol) Which breaks down into two molecules of 3-phosphoglyceric acid (PGA) and it is the first stable product of dark reaction (C3 Cycle). An increase in oxygen concentration decreases photosynthesis and the phenomenon is called Warburg effect. Another way to prevent getting this page in the future is to use Privacy Pass. The quantum yield from the two combined beams was found to be greater than the effect of both beams when used separately. Photosynthesis Definition. They carry energy and the energy contained in a photon is termed as quantum. To release one molecule of oxygen, two molecules of water are required. This number of chlorophyll molecules was called the chlorophyll unit but the name was subsequently changed to photosynthetic unit and later it was designated as Quantasome by Park and Biggins (1964). Give a brief description of the process of synthesis of food in green plants. The splitting of water during photosynthesis is called Photolysis of water. They release sulphur. The assimilation number of variegated variety of a species was found to be higher than the green leaves variety. Completing the CAPTCHA proves you are a human and gives you temporary access to the web property. The energy associated with each photon is called Quantum. Oxygen is important in (a) efficient utilization and complete breakdown of respiratory substrate and (b) formation of ozone in stratosphere that filters out and stops harmful UV radiations in reaching earth. From the total absorption, 4% light is absorbed by the chlorophyll. It has been experimentally proved that an increase in carbon dioxide content of the air up to about one per cent will produce a corresponding increase in photosynthesis provided the intensity of light is also increased. In his experiment Neil used green sulphur bacteria which do not release oxygen during photosynthesis. In the first step, energy from light is stored in the bonds of adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate . In stroma are embedded a number of flattened membranous sacs known as thylakoids. Q4 Give a brief description of the process of synthesis of food in green plants. Before sharing your knowledge on this site, please read the following pages: 1. A metabolic process is conditioned by a number of factors. The energy released by electron is actually used for pumping H+ ions across the thylakoid membrane. (ii) Sudden increased evolution of CO2 when an illuminated green plant is transferred to dark. O2 is not a limiting factor of photosynthesis. These processes are associated with two groups of photosynthetic pigments called as Pigment system I (Photoact I or Photosystem I) and Pigment system II (Photoact II or Photosystem II). It contains pigments chlorophyll a 660, chlorophyll a 670, chlorophyll a 680, chlorophyll a 690, chlorophyll a 700. Accumulation of food in the chloroplasts reduces the rate of photosynthesis. Major types of chlorophylls are known to exist in plants and photosynthetic bacteria viz., Chlorophyll a, b, c, d and e, Bacteriochlorophyll a, b and g, and Chlorobium chlorophyll (Bacterio viridin). Instead, it evolved CO2 and absorbed O2 from its environment. Engelmann (1882) performed an experiment with the freshwater, multicellular filamentous green alga spirogyra. Cloudflare Ray ID: 60e9b4053e03072a This is a unique feature of these succulent plants where they photosynthesis without wasting much of water. The core complex consists of reaction centre associated with proteins and also electon donors and acceptors. The leaves have a green pigment called chlorophyll. 6. Give a brief description of the carbon cycle, including photosynthesis, respiration and combustion. The process through which plants make their own food i.e. It is attach to one of the pyrrole ring. (iii) Some workers have shown that the pace of reaction can be controlled simultaneously by two or more factors. The other intermediate components of electron transport chain viz., PQ (plasto quinone) and PC (plastocyanin) act as mobile electron carriers between two pigment systems. 3. OBJECTIVES 1. There are two important types of phycobilins-Phycoerythrin (Red) and Phycocyanin (Blue). CAM plants increase their water-use efficiency, and secondly through its enzyme PEP carboxylase, they are adapted to extreme hot climates. The ATPase enzyme consists of two parts: one called the F0 is embedded in the membrane and forms a trans-membrane channel that carries out facilitated diffusion of protons across the membrane. Stomata of Crassulacean plants remain open at night. Blackmann (1905) pointed out that the process of photosynthesis consists of two phases: (1) Light reaction or Light phase or Light-dependent phase or Photochemical phase. The expelled electron passes through a series of carriers including X (a special chlorophyll molecule), FeS, ferredoxin, plastoquinone, cytochrome b- f complex and plastocyanin before returning to photo Centre. of light per day it favours good photosynthesis. Photosynthetic pigments occur in thylakoid membranes. Yeast: Origin, Reproduction, Life Cycle and Growth Requirements | Industrial Microbiology, How is Bread Made Step by Step? (vi) CO2 acceptor RUBP (5c) is present but absence of PEPA (3C). While the PGA is used up in the Calvin cycle, the phosphoglycolate is dephosphorylated to form glycolate in the chloroplast (Fig. (i) Oxygenic photosynthesis (with evolution of O2) takes place in green eukaryotes and cyanobacteria (blue-green algae). in traces. Electron released during photolysis of water is picked up by reaction centre of PS-II, called P680. In mesophyll cells, CO2 combines with a phosphorylated 5-carbon sugar, called Ribulose bisphosphate (or RuBP). β—carotene on hydrolysis gives Vitamin A. Xanthophylls are yellow coloured oxygen containing carotenoids and are most abundant in nature. The extruded electron passes through FRS ferredoxin, and NADP -reductase which combines it with NADP+ for becoming reduced through H+ releasing during photolysis to form NADPH2. Show with the help of a sketch that the plants are the ultimate source of food. ATPase has a channel that allows diffusion of protons back across the membrane; this releases enough energy to activate ATPase enzyme that catalyzes the formation of ATP. Peroxisome, the actual site of photorespiration, contains enzymes like glycolate oxydase, glutamate glyoxalate aminotransferase, peroxidase and catalase enzymes. 6.9). Chemistry for Biologists resources aim to help you understand the chemistry and chemical principles that underlie a good deal of biology. Photosynthesis. Photosynthesis is a biological process utilized by all green plants to synthesize their own nutrients. If the temperature is greater than about 30°C, the rate of photosynthesis abruptly falls due to thermal inactivation of enzymes. Log in. The visible spectrum ranges from 390 nm to 760 nm (3900 – 7600A), however, the plant life is affected by wave length ranging from 300 – 780 nm. Part of it is reflected, a part transmitted and a part absorbed. The same gets stored in the organic food as bonds between different atoms. 2. To explain it further, say at a given time, only the factor that is most limiting among all will determine the rate of photosynthesis. CAM plants can also obtain a CO2 compensation point of zero at night and in this way accomplish a steeper gradient for CO2 uptake compared to C3 plants. C4 plants are better photosynthesizes. When both are provided in sufficient quantity, the rate of photosynthesis rose initially but again reached a peak. Solution 1 Show Solution Photosynthesis is defined as the process in which the chlorophyll-containing plant cells synthesise food in the form of carbohydrates, using carbon dioxide and water in the presence of solar energy. To synthesize one glucose molecule Calvin cycle requires 6CO2, 18 ATP and 12 NADPH2. The minimum cardinal value is that magnitudes of a factor below which the metabolic process cannot proceed. The explanation of this problem lies in the phenomenon of photorespiration. What is the connecting substance between light reaction and dark reaction? 5. Chlorophyll a 700 or P700 is the reaction centre of PS I. PS II is found in thylakoid membrane and it contains pigments as chlorophyll b 650, chlorophyll a 660, chlorophyll a 670, chlorophyll a 678, chlorophyll a 680 – 690 and phycobillins. The process of photosynthesis is similar to that of C4 plants but instead of spatial separation of initial PEPcase fixation and final Rubisco fixation of CO2, the two steps occur in the same cells (in the stroma of mesophyll chloroplasts) but at different times, night and day, e.g., Sedum, Kalanchoe, Opuntia, Pineapple (Fig. Can we, hence, say that calling the biosynthetic phase as the dark reaction is a misnomer? (B) Dark Reaction (C3 cycle) takes place in stroma of chloroplast. Join now. The serine is taken up by the peroxisome, and through a series of reactions, is converted to glycerate. He provided light of low intensity (say 150 foot candles) and found photosynthesis to occur. Distinguish between a parasite and a saprotroph. During evolution three different ecological variants have evolved with different CO2 incorporation mechanism: C3, C4 and CAM plants. The energy content of a quantum is related to its wave length. They perform acidification or dark fixation of CO2 during night and de-acidification during day time to release carbon dioxide for actual photosynthesis. This process takes place in the cells containing the green pigment chlorophyll. 4. The process by which plants make food is named as photosynthesis. The extruded electron has an energy equivalent to 23 kcal/mole. Oceans contain 11 x 1014 (110,000 billion) tonnes of carbon dioxide. Photosynthesis is the process by which plants and some microorganisms make substances like carbohydrates. For example, chlorophyll absorbs blue and red light. This energy is used to synthesise (prepare) food from carbon dioxide and water. Chlorophyll a is found in all photosynthetic plants except photosynthetic bacteria. The source of light for photosynthesis is sunlight. Effect of light intensity varies from plant to plant, e.g., more in heliophytes (sun loving plants) and less in sciophytes (shade loving plants). Dimorphic (two morphologically distinct type) chloroplasts occur in C4 plants (Fig. The environmental factors are light, CO2, temperature, soil, water, nutrients etc. Mn, Ca, and CI ions play prominent role in the photolysis of water. It has been observed that in most of the cases the quantum requirement is 8. Carotenes are pure hydrocarbons, red or orange in colour and their chemical formula is – C40H56 Some of the common carotenes are -α, β, γ and δ carotenes, Phytotene, Neurosporene, Lycopene (Red pigment found in ripe tomato). All useful plant products are derived from the process of photosynthesis, e.g., timber, rubber, resins, drugs, oils, fibers, etc. A plant can not survive for long at compensation point because there is net lose of organic matter due to respiration of non-green organs and dark respiration. All photosynthetic pigments have the property of fluorescence. These plants are ecologically significant because they can reduce rate of transpiration during day time, and are well adapted to dry and hot habitats. Porphyrin consists of tetrapyrrole rings and central core of Mg. Phytol tail is side chain of hydrocarbon. Share 0. Carotenoids are present both in PS II and PS I. PS I is associated with both cyclic and non-cyclic photophosphorylation, but PS II is associated with only non-cyclic photophosphorylation. The electron carrying extra energy may be expelled from the molecule and is consumed in some other chemical reactions and a fresh normal electron returns to the molecule. Photosynthesis is the biochemical pathway which converts the energy of light into the bonds of glucose molecules. When P680 or P700 (special type of chlorophyll a) of two pigment systems receives quantum of light then it becomes excited and releases electrons. Calvin cycle completes in 4 major phases: 3. 6.13). They add CO2 into the environment and it can stay there for hundreds of years. 6.13). What is the significance of transpiration? 6.16). Photosynthesis is defined as the process in which the chlorophyll-containing plant cells synthesise food in the form of carbohydrates, using carbon dioxide and water in the presence of solar energy. Warburg observed that when intermittent light (flashes of light) of about 1/16 seconds were given to green algae (Chlorella vulgaris and Scenedesmus obliquus), the photosynthetic yield per second was higher as compared to the continuous supply of same intensity of light. The process is also known as carbon fixation process, as it produces carbon compounds which store chemical energy meant to be used in cell growth. (a) Cyclic Photophosphorylation (Fig. Ground substance of chloroplast is filled with a hydrophilic matrix known as stroma. (Fig. If you are on a personal connection, like at home, you can run an anti-virus scan on your device to make sure it is not infected with malware. Differences between Respiration and Photosynthesis, 4 Main Stages of Cellular Reaction in Plants | Metabolic Engineering, Role of Phosphorus in Energy Trapping and Transfer | Respiration. This is the biosynthetic phase of photosynthesis. (2) Dark reaction or Dark phase or Light independent phase or Biochemical phase. Answer Now and help others. 1. Which part of the male reproductive system store the sperm? Glycine enters mitochondria where two molecules of glycine (4 carbons) give rise to one molecule of serine (3 carbon) and one CO2 (one carbon). Kortschak, Hartt and Burr (1965) reported that rapidly photosynthesizing sugarcane leaves produced a 4-C compound like aspartic acid and malic acid as a result of CO2 – fixation. Excess of O2 may become inhibitory for the process. ATP synthesis is not direct. For a complete plant, rate of photosynthesis increases with increase in light intensity, except under very high light intensity where phenomenon of Solarization’ occurs, (i.e., photo-oxidation of different cellular components including chlorophyll). It is metabolized. Join now. It is thus possible to divide a description of photosynthesis into those reactions associated with the Calvin cycle and the fixation of carbon dioxide, and those reactions (i.e., capture of light by pigments, electron transport, photophosphorylation) which are directly driven by light. (ii) An oxygenic photosynthesis (without the evolution of O2) takes place in photosynthetic bacteria. Liebig (1843) proposed law of minimum which states that the rate of a process is limited by the pace (rapidity) of the slowest factor. It is of two types: The normal state of the molecule is called as ground state or singlet state. Non-cyclic photophosphorylation is carried out in collaboration of both photo system I and II. This confirms that one phase of photosynthesis is independent of light. There is no photorespiration in these plants. This process does not directly depend on the presence of light but is dependent on the products of the light reaction, i.e., ATP and NADPH, besides CO2 and H2O. The pathway by which all photosynthetic eukaryotic organisms ultimately incorporate CO2 into carbohydrate is known as carbon fixation or photosynthetic carbon reduction (PCR.) The gradient is broken down due to the movement of protons across the membrane to the stroma through the trans membrane channel of the F0 of the ATPase. C4 plants are better adapted to environmental stress than C3 plants. Respiration that is initiated in chloroplasts under light conditions is called photorespiration. Photosynthesis is affected by both environmental and genetic (internal) factors. This process is termed as ‘Photosynthesis’. Ask your question. Reactions taking place in mesophyll cells are stated below: (1st carboxylation). Malic acid is the end product of dark fixation of CO2. Intensity of light can be measured by lux meter. During light reaction, oxygen is evolved and assimilatory power (ATP and NADPH2) are formed. The process of photosynthesis occurs in two steps. Along with electrons that come from the acceptor of electrons of PS I, protons are necessary for the reduction of NADP+ to NADPH+ H+ .These protons are also removed from the stroma. When an electron of a molecule absorbs a quantum of light it is raised to a higher level of energy a state called Excited Second Singlet State. Give a brief description of the process of synthesis of food in green plants. The evolution of oxygen from water was also confirmed by Ruben, Randall, Hassid and Kamen (1941) using heavy isotope (O18) in green alga Chlorella. The value of light compensation point is 2.5 -100 ft. candles for shade plants and 100-400 ft. candles for sun plants. It is that value or point in light intensity and atmospheric CO2 concentration when the rate of photosynthesis is just equivalent to the rate of respiration in the photosynthetic organs so that there is no net gaseous exchange. 3. Stroma of chloroplast carries both PEPCO and RUBISCO. Other accessory pigments and chlorophyll molecules are light gatherers or antenna molecules. Pdf File Share Your PPT File Your PDF File Share Your PDF File Share Word... Future is to harvest light energy ( solar energy into chemical energy and used to form PGA powers given! Of lipid and proteins enzyme, called P680 this page in the while... 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